Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Migration von ZORA auf die Software DSpace

ZORA will change to a new software on 8th September 2025. Please note: deadline for new submissions is 21th July 2025!

Information & dates for training courses can be found here: Information on Software Migration.

Differential maturation of GIRK2-expressing neurons in the mouse cerebellum

Aguado, Carolina; Fernández-Alacid, Laura; Cabañero, María José; Yanagawa, Yuchio; Schilling, Karl; Watanabe, Masahiko; Fritschy, Jean-Marc; Luján, Rafael (2013). Differential maturation of GIRK2-expressing neurons in the mouse cerebellum. Journal of Chemical Neuroanatomy, 47:79-89.

Abstract

The cerebellar cortex is among the brain regions showing the highest expression levels of G-protein-gated inwardly-rectifying potassium (GIRK/Kir3) channels. Despite their critical contribution in modulating neuronal excitability during development and adult, the spatiotemporal expression of specific GIRK subunits in identified cerebellar neuron populations is unresolved. To characterize this onset of expression, we examined the GIRK2 protein expression in mouse cerebellum by western blot, light microscopy immunohistochemistry and immunofluorescence during perinatal development. Using western blots, GIRK2 expression was low at birth but reach its maximum at P5 before decreasing gradually to adult levels. Immunohistochemical localization indicated that GIRK2 is expressed in granule cells from early stages of development. At the embryonic stage, immunofluorescence techniques for the transcription factor Pax6 allowed to demonstrate that GIRK2 is expressed in granule cell precursors. This GIRK2 expression in granule cells continued throughout postnatal development and adulthood. In addition, the expression of Pax2-GFP allowed selective visualization of Golgi cells during pre- and postnatal development. We could not detect co-expression of Pax2-GFP and GIRK2 during prenatal and early postnatal development, but only at post-migratory stages of Golgi cells, once they are morphologically differentiated and located at the granule cell layer. In the adult cerebellum, we performed a detailed characterization on the expression of GIRK2 in different subpopulations of Golgi cells, using metabotropic glutamate receptor 2 (mGlu(2)) and neurogranin as markers, in GlyT2-GFP and GAD67-GFP mice, and showed that GIRK2 is present in at least four morphological and neurochemical non-overlapping populations of Golgi cells. Altogether, these findings shed new light on the developmental regulation of GIRK channels in the cerebellum, and the main expression in granule cells during perinatal development support the idea that GIRK2 may provide a significant route for modulating different aspects of cerebellar development.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Pharmacology and Toxicology
07 Faculty of Science > Institute of Pharmacology and Toxicology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > Cellular and Molecular Neuroscience
Language:English
Date:January 2013
Deposited On:21 Mar 2013 16:12
Last Modified:08 Jul 2025 01:41
Publisher:Elsevier
ISSN:0891-0618
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.jchemneu.2012.11.001
PubMed ID:23261870

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
9 citations in Web of Science®
9 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 21 Mar 2013
0 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications