Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Automatic Generation of Predictive Dynamic Models Reveals Nuclear Phosphorylation as the Key Msn2 Control Mechanism

Sunnåker, Mikael; Zamora-Sillero, Elias; Dechant, Reinhard; Ludwig, Christina; Busetto, Alberto Giovanni; Wagner, Andreas; Stelling, Joerg (2013). Automatic Generation of Predictive Dynamic Models Reveals Nuclear Phosphorylation as the Key Msn2 Control Mechanism. Science Signaling, 6(277):ra41.

Abstract

Predictive dynamical models are critical for the analysis of complex biological systems. However, methods to systematically develop and discriminate among systems biology models are still lacking. Here, we describe a computational method that incorporates all hypothetical mechanisms about the architecture of a biological system into a single model, and automatically generates a set of simpler models compatible with observational data. As a proof-of-principle, we analyzed the dynamic control of the transcription factor Msn2 in Saccharomyces cerevisiae, specifically the short-term mechanisms mediating the cells’ recovery after release from starvation stress. Our method determined that twelve out of 192 possible models were compatible with available Msn2 localization data. Iterations between model predictions and rationally designed phosphoproteomics and imaging experiments identified a single circuit topology with a relative probability of 99% among the 192 models. Model analysis revealed that the coupling of dynamic phenomena in Msn2 phosphorylation and transport could lead to efficient stress-response signaling by establishing a rate-of-change sensor. Similar principles could apply to mammalian stress-response pathways. Systematic construction of dynamic models may yield detailed insight into non-obvious molecular mechanisms.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > YeastX
Special Collections > SystemsX.ch > Research, Technology and Development Projects
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Biochemistry
Life Sciences > Molecular Biology
Life Sciences > Cell Biology
Language:English
Date:May 2013
Deposited On:24 Jun 2013 17:52
Last Modified:09 Nov 2024 02:38
Publisher:American Association for the Advancement of Science
ISSN:1945-0877
OA Status:Closed
Publisher DOI:https://doi.org/10.1126/scisignal.2003621

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
41 citations in Web of Science®
43 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

78 downloads since deposited on 24 Jun 2013
11 downloads since 12 months
Detailed statistics

Authors, Affiliations, Collaborations

Similar Publications