Gallo, Sofia; Furler, Maya; Sigel, Roland K O (2005). In vitro Transcription and Purification of RNAs of Different Size. CHIMIA International Journal for Chemistry, 59(11):812-816.
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegant way to obtain highly pure and uniform RNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. Here we describe the different strategies applied and optimized in our laboratory to enzymatically synthesize RNAs as necessary when working at the interface of bioinorganic chemistry, coordination chemistry, RNA biochemistry and structural biology.
In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegant way to obtain highly pure and uniform RNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. Here we describe the different strategies applied and optimized in our laboratory to enzymatically synthesize RNAs as necessary when working at the interface of bioinorganic chemistry, coordination chemistry, RNA biochemistry and structural biology.
| Item Type: | Journal Article, refereed, further contribution |
|---|---|
| Communities & Collections: | 07 Faculty of Science > Department of Chemistry |
| Dewey Decimal Classification: | 540 Chemistry |
| Scopus Subject Areas: | Physical Sciences > General Chemistry |
| Language: | English |
| Date: | November 2005 |
| Deposited On: | 08 Aug 2013 07:47 |
| Last Modified: | 23 Jun 2022 08:14 |
| Publisher: | Swiss Chemical Society |
| ISSN: | 0009-4293 |
| Additional Information: | Copyright ©Swiss Chemical Society: CHIMIA, 59(11):812-816, 2005 |
| OA Status: | Gold |
| Free access at: | Publisher DOI. An embargo period may apply. |
| Publisher DOI: | https://doi.org/10.2533/000942905777675589 |
Gallo, Sofia