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Connecting μ-fluidics to electron microscopy


Abstract

A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-­dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-­pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

Abstract

A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-­dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-­pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

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Item Type:Journal Article, refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > CINA
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Structural Biology
Language:English
Date:2012
Deposited On:28 Aug 2013 11:06
Last Modified:24 Jan 2022 01:26
Publisher:Elsevier
ISSN:1047-8477
OA Status:Green
Publisher DOI:https://doi.org/10.1016/j.jsb.2011.11.001