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Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast.

Fendt, Sarah-Maria; Oliveira, Ana Paula; Christen, Stefan; Picotti, Paola; Dechant, Reinhard Christoph; Sauer, Uwe (2010). Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast. Molecular Systems Biology, 6:432.

Abstract

Which transcription factors control the distribution of metabolic fluxes under a given condition? We address this question by systematically quantifying metabolic fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae under five growth conditions. While most knockouts did not affect fluxes, we identified 42 condition-dependent interactions that were mediated by a total of 23 transcription factors that control almost exclusively the cellular decision between respiration and fermentation. This relatively sparse, condition-specific network of active metabolic control contrasts with the much larger gene regulation network inferred from expression and DNA binding data. Based on protein and transcript analyses in key mutants, we identified three enzymes in the tricarboxylic acid cycle as the key targets of this transcriptional control. For the transcription factor Gcn4, we demonstrate that this control is mediated through the PKA and Snf1 signaling cascade. The discrepancy between flux response predictions, based on the known regulatory network architecture and our functional (13)C-data, demonstrates the importance of identifying and quantifying the extent to which regulatory effectors alter cellular functions.

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > YeastX
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > General Biochemistry, Genetics and Molecular Biology
Life Sciences > General Immunology and Microbiology
Life Sciences > General Agricultural and Biological Sciences
Physical Sciences > Applied Mathematics
Language:English
Date:2010
Deposited On:11 Sep 2013 17:24
Last Modified:28 Oct 2024 04:31
Publisher:European Molecular Biology Organization
ISSN:1744-4292
OA Status:Gold
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1038/msb.2010.91
PubMed ID:21119627
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