Navigation auf zora.uzh.ch

Search ZORA

ZORA (Zurich Open Repository and Archive)

Migration von ZORA auf die Software DSpace

ZORA will change to a new software on 8th September 2025. Please note: deadline for new submissions is 21th July 2025!

Information & dates for training courses can be found here: Information on Software Migration.

The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta.

Henneke, G; Raffin, J P; Ferrari, E; Jónsson, Z O; Dietrich, J; Hübscher, U (2000). The PCNA from Thermococcus fumicolans functionally interacts with DNA polymerase delta. Biochemical and Biophysical Research Communications (BBRC), 276(2):600-6.

Abstract

We have cloned the gene encoding proliferating cell nuclear antigen (PCNA) from the hyperthermophilic euryarchaeote Thermococcus fumicolans (Tfu). Tfu PCNA contains 250 amino acids with a calculated M(r) of 28,000 and is 26% identical to human PCNA. Next, Tfu PCNA was overexpressed in Escherichia coli and it showed an apparent molecular mass of 33.5 kDa. The purified Tfu PCNA was tested first with recombinant Tfu DNA polymerase I (Tfu pol) and second with calf thymus DNA polymerase delta (pol delta). When tested with the homologous Tfu pol on bacteriophage lambda DNA, large amounts of Tfu PCNA were required to obtain two- to threefold stimulation. Surprisingly, however, Tfu PCNA was much more efficient than human PCNA in stimulating calf thymus pol delta. Our data suggest that PCNA has been functionally conserved not only within eukaryotes but also from hyperthermophilic euryarchaeotes to mammals.

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:05 Vetsuisse Faculty > Veterinärwissenschaftliches Institut > Department of Molecular Mechanisms of Disease
07 Faculty of Science > Department of Molecular Mechanisms of Disease
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Biophysics
Life Sciences > Biochemistry
Life Sciences > Molecular Biology
Life Sciences > Cell Biology
Language:English
Date:24 September 2000
Deposited On:11 Feb 2008 12:18
Last Modified:01 Jul 2025 03:36
Publisher:Elsevier
ISSN:0006-291X
OA Status:Closed
Publisher DOI:https://doi.org/10.1006/bbrc.2000.3481
Related URLs:http://www.sciencedirect.com/science/journal/0006291X
PubMed ID:11027519
Full text not available from this repository.

Metadata Export

Statistics

Citations

Dimensions.ai Metrics
16 citations in Web of Science®
21 citations in Scopus®
Google Scholar™

Altmetrics

Authors, Affiliations, Collaborations

Similar Publications