Hybridization can occur under natural conditions among well-differentiated species and may affect the developmental stability of hybrids. In the present study, we investigated the effect of interspecific hybridization between Bombina bombina and B. variegata on fluctuating asymmetry (FA) of forelimb, femur, tibia and foot. The hybrids did not show higher levels of FA compared to the parental populations for either investigated traits. This suggested that the effect of hybridization on FA in the analyzed traits is negligible or overwhelmed by other factors. A significantly increased FA was found in the B. bombina populations when compared to B. variegata, which can be attributed to low pressure of natural selection in these populations.