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MALDI-TOF MS for identification of porcine Brachyspira species


Prohaska, Sarah; Pflueger, V; Scherrer, Simone; Frei, Daniel; Lehmann, Andreas; Wittenbrink, Max Michael; Huber, Helen (2014). MALDI-TOF MS for identification of porcine Brachyspira species. Letters in Applied Microbiology, 58(3):292-298.

Abstract

Significance and Impact of the Study: Current methods for the discrimination of pathogenic Brachyspira hyodysenteriae and Brachyspira pilosicoli from Brachyspira species with low pathogenic potential have proven to be laborious and time-consuming and are therefore not suitable for routine diagnostics. This study describes the evaluation of MALDI-TOF MS for the identification of different porcine Brachyspira species in routine diagnostic laboratories. The results suggest that MALDI-TOF MS is an effective method for the identification of porcine Brachyspira spp. and accelerates diagnosis of swine dysentery and porcine intestinal spirochaetosis.

Abstract

Significance and Impact of the Study: Current methods for the discrimination of pathogenic Brachyspira hyodysenteriae and Brachyspira pilosicoli from Brachyspira species with low pathogenic potential have proven to be laborious and time-consuming and are therefore not suitable for routine diagnostics. This study describes the evaluation of MALDI-TOF MS for the identification of different porcine Brachyspira species in routine diagnostic laboratories. The results suggest that MALDI-TOF MS is an effective method for the identification of porcine Brachyspira spp. and accelerates diagnosis of swine dysentery and porcine intestinal spirochaetosis.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
Scopus Subject Areas:Life Sciences > Applied Microbiology and Biotechnology
Uncontrolled Keywords:Brachyspira, MALDI-TOF MS, pigs, porcine intestinal spirochaetosis, swine dysentery.
Language:English
Date:2014
Deposited On:20 Jan 2014 10:34
Last Modified:30 Jul 2020 11:48
Publisher:Wiley-Blackwell
ISSN:0266-8254
OA Status:Closed
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1111/lam.12189

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