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Zhou, Yueyang; Yu, Jing; Lei, Xiling; Wu, Jianying; Niu, Qi; Zhang, Yixuan; Liu, Hong; Christen, Philipp; Gehring, Heinz; Wu, Fang (2013). High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway. Chemical Communications, 49(100):11782-11784.
We report a high-throughput assay for H2S-producing enzymes, which is based on a newly designed tandem-well plate. Screening of 21,599 agents identified several potent inhibitors of cystathionine β-synthase and cystathionine γ-lyase, the two key enzymes generating H2S in mammals, with IC50 values in the low two-digit micromolar range.
| Item Type: | Journal Article, refereed, original work |
|---|---|
| Communities & Collections: | 04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry |
| Dewey Decimal Classification: | 570 Life sciences; biology |
| Scopus Subject Areas: | Physical Sciences > Catalysis
Physical Sciences > Electronic, Optical and Magnetic Materials Physical Sciences > Ceramics and Composites Physical Sciences > General Chemistry Physical Sciences > Surfaces, Coatings and Films Physical Sciences > Metals and Alloys Physical Sciences > Materials Chemistry |
| Language: | English |
| Date: | 2013 |
| Deposited On: | 23 Jan 2014 12:31 |
| Last Modified: | 10 Mar 2025 02:41 |
| Publisher: | RSC Publishing |
| ISSN: | 1359-7345 |
| OA Status: | Closed |
| Free access at: | Publisher DOI. An embargo period may apply. |
| Publisher DOI: | https://doi.org/10.1039/c3cc46719h |
| PubMed ID: | 24213681 |
Zhou, Yueyang