Two-dimensional visualization of multicolor FISH-generated data as a helpful tool for the analysis and understanding of cytogenetic and chromosomal alterations in melanocytic lesions

Abstract

For the evaluation of data generated by multicolor fluorescence in situ hybridization (FISH), we present here a synergistic approach that integrates the 3 most commonly used numerical algorithms in conjunction with 2 newly devised graphic tools for data visualization, namely "signal curves" and "rhombic heat maps." These two graphic tools provide information additional to the currently used numerical algorithms and thus facilitate the recognition and compensation of inherent errors that occur with the numerical method.