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Conditional gene targeting: a refined method for genetic studies in neurosciences


Beil, Jane; Mansuy, Isabelle M; Buch, Thorsten (2013). Conditional gene targeting: a refined method for genetic studies in neurosciences. In: Nguyen, Peter. Multidisciplinary Tools for Investigating Synaptic Plasticity. United States: Springer (Bücher), 3-41.

Abstract

Conditional mutagenesis in mice is a key approach in neurosciences that makes it possible to investigate the functions of defined genes within certain neural subpopulations. The approach is based on the combination of transgenic expression of a recombinase with targeted candidate genes containing recognition sequences of this recombinase generated by gene targeting. Here we describe three major recombination systems, the Cre/loxP, Flp/FRT, and Dre/rox, and present a procedure that allows the generation of target alleles for these systems. We provide detailed protocols for the culture and transfection of embryonic stem (ES) cells and for the identification and confirmation of homologous recombinants. This chapter also provides an overview of recent developments such as gene targeting in oocytes and discusses future perspectives.

Abstract

Conditional mutagenesis in mice is a key approach in neurosciences that makes it possible to investigate the functions of defined genes within certain neural subpopulations. The approach is based on the combination of transgenic expression of a recombinase with targeted candidate genes containing recognition sequences of this recombinase generated by gene targeting. Here we describe three major recombination systems, the Cre/loxP, Flp/FRT, and Dre/rox, and present a procedure that allows the generation of target alleles for these systems. We provide detailed protocols for the culture and transfection of embryonic stem (ES) cells and for the identification and confirmation of homologous recombinants. This chapter also provides an overview of recent developments such as gene targeting in oocytes and discusses future perspectives.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Life Sciences > General Neuroscience
Life Sciences > General Biochemistry, Genetics and Molecular Biology
Life Sciences > General Pharmacology, Toxicology and Pharmaceutics
Health Sciences > Psychiatry and Mental Health
Language:English
Date:2013
Deposited On:25 Feb 2014 16:46
Last Modified:02 Feb 2024 08:06
Publisher:Springer (Bücher)
Series Name:Neuromethods
Number:81
ISSN:0893-2336
ISBN:978-1-62703-516-3
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-62703-517-0_1
Other Identification Number:Online ISBN: 978-1-62703-517-0