Moorish gecko Tarentola mauritanica and Turkish gecko Hemydactilus turcicus are among the most widely distributed lizards of the Mediterranean basin. However, records for these species at altitudes above 400 m a.s.l. are few. In this paper, we document the presence of these species in a hilly area in the northernmost part of the Province of Grosseto (Tuscany, Central Italy). In particular, Moorish geckoes were found up to 609 m a.s.l. since early 2000s. First individuals were possibly introduced with transport of building materials: the average increase in winter temperature of the past recent years might have helped the establishment of breeding populations.