Engineered fibrin matrices for functional display of cell membrane-bound growth factor-like activities: study of angiogenic signaling by ephrin-B2

Zisch, A H; Zeisberger, S M; Ehrbar, M; Djonov, V; Weber, C C; Ziemiecki, A; Pasquale, E B; Hubbell, J A

doi:10.1016/j.biomaterials.2003.10.015

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Zisch, A. H., Zeisberger, S. M., Ehrbar, M., Djonov, V., Weber, C. C., Ziemiecki, A., Pasquale, E. B., & Hubbell, J. A. (2004). Engineered fibrin matrices for functional display of cell membrane-bound growth factor-like activities: study of angiogenic signaling by ephrin-B2. Biomaterials, 25, 3245–3257. https://doi.org/10.1016/j.biomaterials.2003.10.015

Abstract

With the rapid increase in approaches to pro- or anti-angiogenic therapy, new and effective methodologies for administration of cell-bound growth factors will be required. We sought to develop the natural hydrogel matrix fibrin as platform for extensive interactions and continuous signaling by the vascular morphogen ephrin-B2 that normally resides in the plasma membrane and requires multivalent presentation for ligation and activation of Eph receptors on apposing endothelial cell surfaces. Using fibrin and protein engineering technolo