Publication: Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility
Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility
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Schmidheini, L., Mathis, N., Marquart, K. F., Rothgangl, T., Kissling, L., Böck, D., Chanez, C., Wang, J. P., Jinek, M., & Schwank, G. (2024). Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility. Nature Chemical Biology, 20, 333–343. https://doi.org/10.1038/s41589-023-01427-x
Abstract
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CRISPR-Cas9 genome engineering is a powerful technology for correcting genetic diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a protospacer adjacent motif (PAM), and in vivo delivery is challenging due to their large size. Here, we use phage-assisted continuous directed evolution to broaden the PAM compatibility of Campylobacter jejuni Cas9 (CjCas9), the smallest Cas9 ortholog characterized to date. The identified variant, termed evoCjCas9, primarily recognizes N${4}$AH and N${5}$
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- Schmidheini, Lukas
- Mathis, Nicolas
- Marquart, Kim Fabiano
- Rothgangl, Tanja
- Kissling, Lucas
- Böck, Desirée
- Chanez, Christelle
- Wang, Jingrui Priscilla
- Jinek, Martin
- Schwank, Gerald
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Schmidheini, L., Mathis, N., Marquart, K. F., Rothgangl, T., Kissling, L., Böck, D., Chanez, C., Wang, J. P., Jinek, M., & Schwank, G. (2024). Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility. Nature Chemical Biology, 20, 333–343. https://doi.org/10.1038/s41589-023-01427-x
