Publication: Correlative super-resolution and electron microscopy to resolve protein localization in zebrafish retina
Correlative super-resolution and electron microscopy to resolve protein localization in zebrafish retina
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Mateos, J. M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S. C. F., Kaech, A., Bachmann-Gagescu, R., & Ziegler, U. (2017). Correlative super-resolution and electron microscopy to resolve protein localization in zebrafish retina. Journal of Visualized Experiments, 129, e56113. https://doi.org/10.3791/56113
Abstract
Abstract
Abstract
We present a method to investigate the subcellular protein localization in the larval zebrafish retina by combining super-resolution light microscopy and scanning electron microscopy. The sub-diffraction limit resolution capabilities of super-resolution light microscopes allow improving the accuracy of the correlated data. Briefly, 110 nanometer thick cryo-sections are transferred to a silicon wafer and, after immunofluorescence staining, are imaged by super-resolution light microscopy. Subsequently, the sections are preserved in meth
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Creators (Authors)
- Barmettler, Gery
- Doehner, Jana
- Ojeda Naharros, Irene
- Guhl, Bruno
- Kaech, Andres
- Ziegler, Urs
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Citations
Mateos, J. M., Barmettler, G., Doehner, J., Ojeda Naharros, I., Guhl, B., Neuhauss, S. C. F., Kaech, A., Bachmann-Gagescu, R., & Ziegler, U. (2017). Correlative super-resolution and electron microscopy to resolve protein localization in zebrafish retina. Journal of Visualized Experiments, 129, e56113. https://doi.org/10.3791/56113
