Publication: Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens
Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens
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de Groot, R., Lüthi, J., Lindsay, H., Holtackers, R., & Pelkmans, L. (2018). Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens. Molecular Systems Biology, 14, e8064. https://doi.org/10.15252/msb.20178064
Abstract
Abstract
Abstract
High-content imaging using automated microscopy and computer vision allows multivariate profiling of single-cell phenotypes. Here, we present methods for the application of the CISPR-Cas9 system in large-scale, image-based, gene perturbation experiments. We show that CRISPR-Cas9-mediated gene perturbation can be achieved in human tissue culture cells in a timeframe that is compatible with image-based phenotyping. We developed a pipeline to construct a large-scale arrayed library of 2,281 sequence-verified CRISPR-Cas9 targeting plasmid
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- de Groot, Reinoud
- Lüthi, Joel
- Lindsay, Helen
- Holtackers, René
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Citations
de Groot, R., Lüthi, J., Lindsay, H., Holtackers, R., & Pelkmans, L. (2018). Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens. Molecular Systems Biology, 14, e8064. https://doi.org/10.15252/msb.20178064
