Publication: Enhanced multiplexing in mass cytometry using osmium and ruthenium tetroxide species
Enhanced multiplexing in mass cytometry using osmium and ruthenium tetroxide species
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Catena, R., Özcan, A., Zivanovic, N., & Bodenmiller, B. (2016). Enhanced multiplexing in mass cytometry using osmium and ruthenium tetroxide species. Cytometry. Part A, 89, 491–497. https://doi.org/10.1002/cyto.a.22848
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Mass cytometry facilitates high-dimensional, quantitative, single-cell analysis. The method for sample multiplexing in mass cytometry, called mass-tag cellular barcoding (MCB), relies on the covalent reaction of bifunctional metal chelators with intracellular proteins. Here, we describe the use of osmium and ruthenium tetroxides (OsO4 and RuO4 ) that bind covalently with fatty acids in the cellular membranes and aromatic amino acids in proteins. Both OsO4 and RuO4 rapidly reacted and allowed for MCB with live cells, crosslinked cells,
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- Catena, Raúl
- Özcan, Alaz
- Zivanovic, Nevena
- Bodenmiller, Bernd
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Catena, R., Özcan, A., Zivanovic, N., & Bodenmiller, B. (2016). Enhanced multiplexing in mass cytometry using osmium and ruthenium tetroxide species. Cytometry. Part A, 89, 491–497. https://doi.org/10.1002/cyto.a.22848