Publication: Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes
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Yin, J.-A., Frick, L., Scheidmann, M. C., Liu, T., Trevisan, C., Dhingra, A., Spinelli, A., Wu, Y., Yao, L., Vena, D. L., Knapp, B., Guo, J., De Cecco, E., Ging, K., Armani, A., Oakeley, E. J., Nigsch, F., Jenzer, J., Haegele, J., … et al. (2024). Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes. Nature Biomedical Engineering, 9(1), 127–148. https://doi.org/10.1038/s41551-024-01278-4
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Arrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libraries can be constructed for the genome-wide ablation (19,936 plasmids) of human protein-coding genes and for their activation and epigenetic silencing (22,442 plasmids), with each plasmid encoding an array of four non-overlapping sg
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Yin, J.-A., Frick, L., Scheidmann, M. C., Liu, T., Trevisan, C., Dhingra, A., Spinelli, A., Wu, Y., Yao, L., Vena, D. L., Knapp, B., Guo, J., De Cecco, E., Ging, K., Armani, A., Oakeley, E. J., Nigsch, F., Jenzer, J., Haegele, J., … et al. (2024). Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes. Nature Biomedical Engineering, 9(1), 127–148. https://doi.org/10.1038/s41551-024-01278-4
