Publication: Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency
Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency
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Chanou, A., Weiβ, M., Holler, K., Sajid, A., Straub, T., Krietsch, J., Sanchi, A., Ummethum, H., Lee, C. S. K., Kruse, E., Trauner, M., Werner, M., Lalonde, M., Lopes, M., Scialdone, A., & Hamperl, S. (2023). Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency. Nucleic Acids Research, 51(22), 12303–12324. https://doi.org/10.1093/nar/gkad1022
Abstract
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Abstract
Stochastic origin activation gives rise to significant cell-to-cell variability in the pattern of genome replication. The molecular basis for heterogeneity in efficiency and timing of individual origins is a long-standing question. Here, we developed Methylation Accessibility of TArgeted Chromatin domain Sequencing (MATAC-Seq) to determine single-molecule chromatin accessibility of four specific genomic loci. MATAC-Seq relies on preferential modification of accessible DNA by methyltransferases combined with Nanopore-Sequencing for dir
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Creators (Authors)
- Chanou, Anna
- Weiβ, Matthias
- Holler, Karoline
- Sajid, Atiqa
- Straub, Tobias
- Krietsch, Jana
- Sanchi, Andrea
- Ummethum, Henning
- Lee, Clare S K
- Kruse, Elisabeth
- Trauner, Manuel
- Werner, Marcel
- Lalonde, Maxime
- Lopes, Massimo
- Scialdone, Antonio
- Hamperl, Stephan
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Chanou, A., Weiβ, M., Holler, K., Sajid, A., Straub, T., Krietsch, J., Sanchi, A., Ummethum, H., Lee, C. S. K., Kruse, E., Trauner, M., Werner, M., Lalonde, M., Lopes, M., Scialdone, A., & Hamperl, S. (2023). Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency. Nucleic Acids Research, 51(22), 12303–12324. https://doi.org/10.1093/nar/gkad1022
