Potential systematic error in laboratory experiments on microbial leakage through filled root canals: an experimental study

Abstract

AIM: To assess the routes of bacterial leakage in a commonly used two-chamber model designed to evaluate root fillings. METHODOLOGY: Fifty-one intact human mandibular premolars with fully developed roots were used. They were left completely intact (n=23), or were accessed, instrumented and either left open (n=5) or root filled with gutta-percha and AH Plus (n=23). All teeth were sealed between two chambers using sticky wax. The apical root aspects were left uncovered. The upper chamber was seeded with Enterococcus faecalis. An enter Show more