Publication: A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheat
A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheat
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Sun, J., Okada, M., Tameshige, T., Shimizu-Inatsugi, R., Akiyama, R., Nagano, A. J., Sese, J., & Shimizu, K. K. (2023). A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheat. NAR Genomics and Bioinformatics, 5(3), lqad067. https://doi.org/10.1093/nargab/lqad067
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Although allopolyploid species are common among natural and crop species, it is not easy to distinguish duplicated genes, known as homeologs, during their genomic analysis. Yet, cost-efficient RNA sequencing (RNA-seq) is to be developed for large-scale transcriptomic studies such as time-series analysis and genome-wide association studies in allopolyploids. In this study, we employed a 3′ RNA-seq utilizing 3′ untranslated regions (UTRs) containing frequent mutations among homeologous genes, compared to coding sequence. Among the 3′ RN
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- Sun, Jianqiang
- Okada, Moeko
- Tameshige, Toshiaki
- Shimizu-Inatsugi, Rie
- Akiyama, Reiko
- Nagano, Atsushi J
- Sese, Jun
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Sun, J., Okada, M., Tameshige, T., Shimizu-Inatsugi, R., Akiyama, R., Nagano, A. J., Sese, J., & Shimizu, K. K. (2023). A low-coverage 3′ RNA-seq to detect homeolog expression in polyploid wheat. NAR Genomics and Bioinformatics, 5(3), lqad067. https://doi.org/10.1093/nargab/lqad067
