Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions

Ramella, Martina; Ribolla, Lucrezia Maria; Surini, Sara; Sala, Kristyna; Tonoli, Diletta; Cioni, Jean-Michel; Rai, Arpan Kumar; Pelkmans, Lucas; de Curtis, Ivan

doi:10.5167/uzh-262406

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Ramella, M., Ribolla, L. M., Surini, S., Sala, K., Tonoli, D., Cioni, J.-M., Rai, A. K., Pelkmans, L., & de Curtis, I. (2024). Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions. IScience, 27(4), 109440. https://doi.org/10.1016/j.isci.2024.109440

Abstract

Plasma membrane-associated platforms (PMAPs) form at specific sites of plasma membrane by scaffolds including ERC1 and Liprin-α1. We identify a mechanism regulating PMAPs assembly, with consequences on motility/invasion. Silencing Ser/Thr kinase DYRK3 in invasive breast cancer cells inhibits their motility and invasive capacity. Similar effects on motility were observed by increasing DYRK3 levels, while kinase-dead DYRK3 had limited effects. DYRK3 overexpression inhibits PMAPs formation and has negative effects on stability of lamelli