The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection

Gomes, Margarida C; Tasrini, Yara; Subramoni, Sujatha; Agnoli, Kirsty; Feliciano, Joana R; Eberl, Leo; Sokol, Pamela; O'Callaghan, David; Vergunst, Annette C

doi:10.5167/uzh-167662

Citations

Citation copied

Gomes, M. C., Tasrini, Y., Subramoni, S., Agnoli, K., Feliciano, J. R., Eberl, L., Sokol, P., O’Callaghan, D., & Vergunst, A. C. (2018). The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection. PLoS Pathogens, 14, e1007473. https://doi.org/10.1371/journal.ppat.1007473

Abstract

The opportunistic pathogen Burkholderia cenocepacia is particularly life-threatening for cystic fibrosis (CF) patients. Chronic lung infections with these bacteria can rapidly develop into fatal pulmonary necrosis and septicaemia. We have recently shown that macrophages are a critical site for replication of B. cenocepacia K56-2 and the induction of fatal pro-inflammatory responses using a zebrafish infection model. Here, we show that ShvR, a LysR-type transcriptional regulator that is important for biofilm formation, rough colony mor