Publication: High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Stierli, S., Sturzenegger, F., Jordaan, W. S., Micheli, S., Martins, J. R., & Sommer, L. (2025). High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples. Journal of Visualized Experiments, 224, online. https://doi.org/10.3791/67696
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Skin injuries initiate a complex regenerative cascade that engages various cell types. Amongst those, peripheral glial cells play a critical role in ensuring the success of the repair process. However, our understanding of these processes remains incomplete due to limitations in current technologies. Therefore, the iterative-bleaching-extends-multiplexity (IBEX) method was employed to characterize changes to the cellular composition of the skin using antibodies directed against proteins expressed by key cell types and structures invol
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Stierli, S., Sturzenegger, F., Jordaan, W. S., Micheli, S., Martins, J. R., & Sommer, L. (2025). High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples. Journal of Visualized Experiments, 224, online. https://doi.org/10.3791/67696