Publication: Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication
Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication
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Gomez-Gonzalez, A., Burkhardt, P., Bauer, M., Suomalainen, M., Mateos, J. M., Loehr, M. O., Luedtke, N. W., & Greber, U. F. (2024). Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication. Science Advances, 10, eadq7483. https://doi.org/10.1126/sciadv.adq7483
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Biomolecular assemblies are fundamental to life and viral disease. The spatiotemporal coordination of viral replication and assembly is largely unknown. Here, we developed a dual-color click chemistry procedure for imaging adenovirus DNA (vDNA) replication in the cell nucleus. Late- but not early-replicated vDNA was packaged into virions. Early-replicated vDNA segregated from the viral replication compartment (VRC). Single object tracking, superresolution microscopy, fluorescence recovery after photobleaching, and correlative light-el
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Gomez-Gonzalez, A., Burkhardt, P., Bauer, M., Suomalainen, M., Mateos, J. M., Loehr, M. O., Luedtke, N. W., & Greber, U. F. (2024). Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication. Science Advances, 10, eadq7483. https://doi.org/10.1126/sciadv.adq7483
