Publication: Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH
Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH
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Alonso-Sáez, L., Balagué, V., Sà, E. L., Sanchez-Pernaute, O., González-Navajas, J. M., Pinhassi, J., Massana, R., Pernthaler, J., Pedrós-Alió, C., & Gasol, J. M. (2007). Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH. FEMS Microbiology Ecology, 60(1), 98–112. https://doi.org/10.1111/j.1574-6941.2006.00276.x
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We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage composition in north-west Mediterranean coastal waters. DGGE analysis indicated that bacterial diversity changed gradually throughout the year, although with a clear distinction of the summer period. Alphaproteobacteria were the dominant group on an annual basis [29% of the DAPI (4′,6-diamidino-2-phenylindole) counts by CARD-FISH, and 70% of the bacter
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Alonso-Sáez, L., Balagué, V., Sà, E. L., Sanchez-Pernaute, O., González-Navajas, J. M., Pinhassi, J., Massana, R., Pernthaler, J., Pedrós-Alió, C., & Gasol, J. M. (2007). Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH. FEMS Microbiology Ecology, 60(1), 98–112. https://doi.org/10.1111/j.1574-6941.2006.00276.x