Publication: Laser-assisted microdissection of plant embryos for transcriptional profiling
Laser-assisted microdissection of plant embryos for transcriptional profiling
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Florez-Rueda, A. M., Waser, L., & Grossniklaus, U. (2020). Laser-assisted microdissection of plant embryos for transcriptional profiling. In M. Bayer (Ed.), Plant Embryogenesis : Methods and Protocols (No. 2122; Issue 2122, pp. 127–139). Springer. https://doi.org/10.1007/978-1-0716-0342-0_10
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Transcriptomic studies have proven powerful and effective as a tool to study the molecular underpinnings of plant development. Still, it remains challenging to disentangle cell- or tissue-specific transcriptomes in complex structures like the plant seed. In particular, the embryo of flowering plants is embedded in the endosperm, a nurturing tissue, which, in turn, is enclosed by the maternal seed coat. Here, we describe laser-assisted microdissection (LAM) to isolate highly pure embryo tissue from whole seeds. This technique is applic
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Florez-Rueda, A. M., Waser, L., & Grossniklaus, U. (2020). Laser-assisted microdissection of plant embryos for transcriptional profiling. In M. Bayer (Ed.), Plant Embryogenesis : Methods and Protocols (No. 2122; Issue 2122, pp. 127–139). Springer. https://doi.org/10.1007/978-1-0716-0342-0_10