Publication:

Laser-assisted microdissection of plant embryos for transcriptional profiling

Date

Date

Date
2020
Book Section
Published version

Citations

Citation copied

Florez-Rueda, A. M., Waser, L., & Grossniklaus, U. (2020). Laser-assisted microdissection of plant embryos for transcriptional profiling. In M. Bayer (Ed.), Plant Embryogenesis : Methods and Protocols (No. 2122; Issue 2122, pp. 127–139). Springer. https://doi.org/10.1007/978-1-0716-0342-0_10

Abstract

Abstract

Abstract

Transcriptomic studies have proven powerful and effective as a tool to study the molecular underpinnings of plant development. Still, it remains challenging to disentangle cell- or tissue-specific transcriptomes in complex structures like the plant seed. In particular, the embryo of flowering plants is embedded in the endosperm, a nurturing tissue, which, in turn, is enclosed by the maternal seed coat. Here, we describe laser-assisted microdissection (LAM) to isolate highly pure embryo tissue from whole seeds. This technique is applic

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100 since deposited on 2021-01-22
94last week
Acq. date: 2025-11-13

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84 since deposited on 2021-01-22
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Acq. date: 2025-11-13

Additional indexing

Creators (Authors)

  • Florez-Rueda, Ana Marcela
    affiliation.icon.alt
  • Waser, Lucas
    affiliation.icon.alt
  • Grossniklaus, Ueli
    affiliation.icon.alt

Editors

  • Bayer, Martin

Title of Book

Title of Book

Title of Book
Plant Embryogenesis : Methods and Protocols

Place of Publication

Place of Publication

Place of Publication
Humana Press, New York

Publisher

Publisher

Publisher

Page range/Item number

Page range/Item number

Page range/Item number
127

Page end

Page end

Page end
139

Item Type

Item Type

Item Type
Book Section

Dewey Decimal Classifikation

Dewey Decimal Classifikation

Dewey Decimal Classifikation

Language

Language

Language
English

Publication date

Publication date

Publication date
2020-01-01

Date available

Date available

Date available
2021-01-22

Series Name

Series Name

Series Name
Methods in Molecular Biology

ISSN or e-ISSN

ISSN or e-ISSN

ISSN or e-ISSN
1064-3745

ISBN or e-ISBN

ISBN or e-ISBN

ISBN or e-ISBN
978-1-0716-0341-3

OA Status

OA Status

OA Status
Green

Metrics

Downloads

100 since deposited on 2021-01-22
94last week
Acq. date: 2025-11-13

Views

84 since deposited on 2021-01-22
83last week
Acq. date: 2025-11-13

Citations

Citation copied

Florez-Rueda, A. M., Waser, L., & Grossniklaus, U. (2020). Laser-assisted microdissection of plant embryos for transcriptional profiling. In M. Bayer (Ed.), Plant Embryogenesis : Methods and Protocols (No. 2122; Issue 2122, pp. 127–139). Springer. https://doi.org/10.1007/978-1-0716-0342-0_10

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