Publication: Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System
Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System
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Lämmermann, H., Nguyen, J., Tamez‐Fernández, J. F., Kuttler, F., Bortoli Chapalay, J., Chambon, M., Turcatti, G., & Rivera‐Fuentes, P. (2025). Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System. Chembiochem, 26(19), e202500494. https://doi.org/10.1002/cbic.202500494
Abstract
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Abstract
Stress response pathways rely on coordinated gene expression regulation. In the endoplasmic reticulum, the unfolded protein response plays an important role in maintaining the homeostasis of protein folding and processing. A chemigenetic reporter assay is developed that allows monitoring of BiP/GRP78 expression, a key regulator of the unfolded protein response. The system is based on the coexpression of the self‐labeling protein HaloTag via an internal ribosome entry site. HaloTag allows for flexibility in labeling color as well as la
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Lämmermann, H., Nguyen, J., Tamez‐Fernández, J. F., Kuttler, F., Bortoli Chapalay, J., Chambon, M., Turcatti, G., & Rivera‐Fuentes, P. (2025). Two‐Color Timestamping of Gene Expression with a Chemigenetic Reporter System. Chembiochem, 26(19), e202500494. https://doi.org/10.1002/cbic.202500494
