Publication: A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury
A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury
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Hamid, A. K., Pastor Arroyo, E. M., Lee, S. S., Wagner, C. A., & Egli-Spichtig, D. (2024). A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury. American Journal of Physiology : Renal Physiology, 326(1), F105–F117. https://doi.org/10.1152/ajprenal.00140.2023
Abstract
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Here, we describe a novel method for the visualization and quantification of renal folic acid (FA) crystals in the rodent FA-induced acute kidney injury (FA-AKI) model. The protocol involves a straightforward histological approach followed by fully automated imaging and quantification steps. Applicability was confirmed by showing that the FA-AKI model is sex-dependent. The method can serve as a tool to aid in characterizing FA-AKI and to control for studies investigating prophylactic therapeutic avenues using FA-AKI.
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- Hamid, Ahmad Kamal
- Pastor Arroyo, Eva Maria
- Lee, Sung Sik
- Wagner, Carsten Alexander
- Egli-Spichtig, Daniela
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Citations
Hamid, A. K., Pastor Arroyo, E. M., Lee, S. S., Wagner, C. A., & Egli-Spichtig, D. (2024). A novel method for automated crystal visualization and quantification in murine folic acid-induced acute kidney injury. American Journal of Physiology : Renal Physiology, 326(1), F105–F117. https://doi.org/10.1152/ajprenal.00140.2023
