Targeted adenovirus-mediated transduction of human T cells in vitro and in vivo

Freitag, Patrick C; Kaulfuss, Meike; Flühler, Lea; Mietz, Juliane; Weiss, Fabian; Brücher, Dominik; Kolibius, Jonas; Hartmann, K Patricia; Smith, Sheena N; Münz, Christian; Chijioke, Obinna; Plückthun, Andreas

doi:10.5167/uzh-233903

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Freitag, P. C., Kaulfuss, M., Flühler, L., Mietz, J., Weiss, F., Brücher, D., Kolibius, J., Hartmann, K. P., Smith, S. N., Münz, C., Chijioke, O., & Plückthun, A. (2023). Targeted adenovirus-mediated transduction of human T cells in vitro and in vivo. Molecular Therapy - Methods & Clinical Development, 29, 120–132. https://doi.org/10.1016/j.omtm.2023.02.012

Abstract

Clinical success in T cell therapy has stimulated widespread efforts to increase safety and potency and to extend this technology to solid tumors. Yet progress in cell therapy remains restricted by the limited payload capacity, specificity of target cell transduction, and transgenic gene expression efficiency of applied viral vectors. This renders complex reprogramming or direct in vivo applications difficult. Here, we developed a synergistic combination of trimeric adapter constructs enabling T cell-directed transduction by the human