Assessment of diagnostic accuracy of a commercial ELISA for the detection of Toxoplasma gondii infection in pigs compared with IFAT, TgSAG1-ELISA and Western blot, using a Bayesian latent class approach

Basso, Walter; Hartnack, Sonja; Pardini, Lais; Maksimov, Pavlo; Koudela, Bretislav; Venturini, Maria C; Schares, Gereon; Sidler, Xaver; Lewis, Fraser I; Deplazes, Peter

doi:10.1016/j.ijpara.2013.02.003

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Basso, W., Hartnack, S., Pardini, L., Maksimov, P., Koudela, B., Venturini, M. C., Schares, G., Sidler, X., Lewis, F. I., & Deplazes, P. (2013). Assessment of diagnostic accuracy of a commercial ELISA for the detection of Toxoplasma gondii infection in pigs compared with IFAT, TgSAG1-ELISA and Western blot, using a Bayesian latent class approach. International Journal for Parasitology, 43(7), 565–570. https://doi.org/10.1016/j.ijpara.2013.02.003

Abstract

Serological methods are the most commonly used diagnostic tools to detect Toxoplasma gondii infections in pigs. In the absence of a readily available 'gold standard', an estimation of diagnostic accuracy is difficult to assess. A commercial ELISA (PrioCHECK® Toxoplasma Ab porcine ELISA, Prionics, Schlieren, Switzerland) for the diagnosis of T. gondii infection in pigs was evaluated in naturally infected animals from two distinct populations; indoor and outdoor living animals. An assessment of diagnostic accuracy, using a Bayesian late