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Architecture and Characteristics of Bacterial Nanotubes


Dubey, Gyanendra P; Malli Mohan, Ganesh Babu; Dubrovsky, Anna; Amen, Triana; Tsipshtein, Shai; Rouvinski, Alex; Rosenberg, Alex; Kaganovich, Daniel; Sherman, Eilon; Medalia, Ohad; Ben-Yehuda, Sigal (2016). Architecture and Characteristics of Bacterial Nanotubes. Developmental Cell, 36(4):453-61.

Abstract

Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as a model organism, we found that at low cell density, nanotubes exhibit remarkable complexity, existing as both intercellular tubes and extending tubes, with the latter frequently surrounding the cells in a "root-like" fashion. Observing nanotube formation in real time showed that these structures are formed in the course of minutes, displaying rapid movements. Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen. Furthermore, we discovered that a conserved calcineurin-like protein, YmdB, presents in nanotubes and is required for both nanotube production and intercellular molecular trade.

Abstract

Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as a model organism, we found that at low cell density, nanotubes exhibit remarkable complexity, existing as both intercellular tubes and extending tubes, with the latter frequently surrounding the cells in a "root-like" fashion. Observing nanotube formation in real time showed that these structures are formed in the course of minutes, displaying rapid movements. Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen. Furthermore, we discovered that a conserved calcineurin-like protein, YmdB, presents in nanotubes and is required for both nanotube production and intercellular molecular trade.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:22 February 2016
Deposited On:18 Jul 2016 12:02
Last Modified:19 Aug 2018 03:42
Publisher:Cell Press (Elsevier)
ISSN:1534-5807
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.devcel.2016.01.013
PubMed ID:26906740
Project Information:
  • : FunderFP7
  • : Grant ID337713
  • : Project TitleDARKSIDE - Harnessing the Dark Side of Protein Folding: Manipulating Aggregation for Recombinant Protein Production
  • : FunderFP7
  • : Grant ID339984
  • : Project TitleBACTERIAL RESPONSE - New Concepts in Bacterial Response to their Surroundings
  • : FunderFP7
  • : Grant ID321993
  • : Project TitlePALM TCR COMPLEXES - Studying the Structure and Dynamics of TCR nucleated Complexes at the single molecule level

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