Header

UZH-Logo

Maintenance Infos

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium


Abstract

A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the “zygotene chromosomal bouquet.” Here, we identified the “zygotene cilium” in oocytes. This cilium provides a cable system for the bouquet machinery and extends throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies and suggests that cilia can control chromosomal dynamics.

Abstract

A hallmark of meiosis is chromosomal pairing, which requires telomere tethering and rotation on the nuclear envelope through microtubules, driving chromosome homology searches. Telomere pulling toward the centrosome forms the “zygotene chromosomal bouquet.” Here, we identified the “zygotene cilium” in oocytes. This cilium provides a cable system for the bouquet machinery and extends throughout the germline cyst. Using zebrafish mutants and live manipulations, we demonstrate that the cilium anchors the centrosome to counterbalance telomere pulling. The cilium is essential for bouquet and synaptonemal complex formation, oogenesis, ovarian development, and fertility. Thus, a cilium represents a conserved player in zebrafish and mouse meiosis, which sheds light on reproductive aspects in ciliopathies and suggests that cilia can control chromosomal dynamics.

Statistics

Citations

Dimensions.ai Metrics
16 citations in Web of Science®
16 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

368 downloads since deposited on 11 Jul 2022
77 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Genetics
07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Scopus Subject Areas:Health Sciences > Multidisciplinary
Uncontrolled Keywords:Animals, Centromere / genetics, Centromere / physiology, Chromosome Pairing* / genetics, Chromosome Pairing* / physiology, Cilia* / physiology, Female Fertility / physiology, Mice Morphogenesis Oocytes* / growth & development, Oogenesis* / genetics, Oogenesis* / physiology, Ovary* / growth & development, Telomere / genetics, Telomere / physiology, Zebrafish / genetics, Zebrafish / physiology
Language:English
Date:17 June 2022
Deposited On:11 Jul 2022 07:16
Last Modified:27 Apr 2024 01:38
Publisher:American Association for the Advancement of Science
ISSN:0036-8075
Additional Information:Funding: This work was supported by the Israel Science Foundation–Singapore National Research Foundation joint research program (grant no. 3291/19 to Y.M.E. and S.R.); the Ben Schender Fund for Outstanding Young Scientists (Y.M.E.); a Dr. Gabrielle Reem-Kayden Scholarship to A.M. and K.L.; a HUJI International PhD Talent Scholarship to V.K.; and an Abisch-Frenkel Scholarship for Scientific Excellence to N.H. ////// This is the author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in [Science / 0036-8075] on [376:6599], DOI: [10.1126/science.abh3104].
OA Status:Green
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1126/science.abh3104
PubMed ID:35549308
  • Content: Accepted Version
  • Language: English